Regenerative Biology
TB-500
N-acetylated thymosin beta-4 residues 17–23
TB-500 fragment is a synthetic N-acetylated heptapeptide corresponding to the LKKTETQ region associated with residues 17–23 of thymosin beta-4. It is used in laboratory research involving peptide identification, actin-associated sequence studies, analytical method development, and experimental cell models. For laboratory research use only. Not for human or veterinary use.
Frequently researched together
Other laboratory research compounds available in our catalog.
Compound information
Technical specifications
What is TB-500?
TB-500 is a synthetic 7-residue peptide whose sequence corresponds to the LKKTETQ region of thymosin beta-4, associated with residues 17 to 23 of that parent protein, supplied in N-acetylated form. It is the short fragment rather than the full-length actin-binding protein, although the catalogue lists Thymosin Beta-4 and TB4 as synonyms for the entry. The material is filed under regenerative biology and offered as a lyophilized powder in a 10 mg vial.
The sequence is written Ac-LKKTETQ, with an acetyl group on the N terminus that removes the free alpha-amino group and shifts both charge and mass relative to the unmodified heptapeptide. The catalogue entry lists the molecular formula C38H68N10O14; a CAS registry number and a molecular weight are not recorded here, so neither is stated. Identity work proceeds from the sequence, the formula and the expected mass given on the certificate for the lot.
Lyophilized material is held at -20 C protected from light, and reconstituted solution at 2 to 8 C for short-term laboratory use, with repeated freeze-thaw cycling avoided. Purity and identity are verified by third-party HPLC and mass spectrometry, with a certificate of analysis per lot. The peptide has been the subject of published laboratory and preclinical research. It is supplied strictly for laboratory research and is not for human or veterinary use.
- Molecular formula
- C38H68N10O14
Stability information
Recommended handling and storage conditions for retaining compound identity and integrity in the laboratory.
Lyophilized
-20°C · Protect from light
Reconstituted
2-8°C
Regulatory information
Development origin and regulatory status, compiled for reference.
Research compound · Not FDA-approved
Frequently asked
Questions about TB-500
What purity is TB-500 supplied at, and is a Certificate of Analysis available?
Yes. Each lot of TB-500 is tested by a third-party laboratory using HPLC for purity, and the certificate of analysis for the current lot is linked on this page and in the certificate library.
How is the identity of TB-500 verified?
Identity is confirmed by mass spectrometry against the expected molecular weight, alongside HPLC retention behaviour. Both results are recorded on the lot certificate.
How should TB-500 be stored on receipt?
Lyophilized: -20°C (Protect from light); Reconstituted: 2-8°C.
Where is TB-500 shipped from?
Orders ship from the United States in discreet, tracked packaging. Shipping is available to US addresses only.
Is TB-500 supplied for research use only?
Yes. TB-500 is sold strictly as a laboratory research material. It is not for human or veterinary use, not for diagnostic procedures, and has not been evaluated by the U.S. Food and Drug Administration. See the research use disclaimer for the full terms.
Answers describe how this material is tested, stored and shipped. They are not guidance on any use, and this compound is not for human or veterinary use.
Sources & references
Published literature in which this compound has been the subject of laboratory or preclinical study. Listed for bibliographic reference only.
Research and educational purposes only. These references are provided for bibliographic context. They are not evidence of safety or efficacy, and nothing here is medical advice or a claim about any use in humans or animals.
Annals of the New York Academy of Sciences
Thymosin beta-4: actin-sequestering protein moonlights to repair
FASEB journal : official publication of the Federation of American Societies for Experimental Biology
Biological activities of thymosin beta4 defined by active sites in short peptide sequences
ACS applied materials & interfaces
Alkaline Phosphatase-Triggered Spatiotemporal Repair of Corneal Injury with TB500 Peptide Hydrogel
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
Simultaneous quantification of TB-500 and its metabolites in in-vitro experiments and rats by UHPLC-Q-Exactive orbitrap MS/MS and their screening by wound healing activities in-vitro
Expert opinion on biological therapy
Thymosin β4 inhibits PDGF-BB induced activation, proliferation, and migration of human hepatic stellate cells via its actin-binding domain
Annals of the New York Academy of Sciences
Subcellular distribution of thymosin beta4
Drug testing and analysis
Synthesis and characterization of the N-terminal acetylated 17-23 fragment of thymosin beta 4 identified in TB-500, a product suspected to possess doping potential
Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society
Thymosin beta 4 and a synthetic peptide containing its actin-binding domain promote dermal wound repair in db/db diabetic mice and in aged mice